Transient expression of chimeric genes delivered into pollen by microprojectile bombardment.

The S-Ribonuclease Gene of Petunia hybrida

To determine the ability of isolated S-locus promoter sequences to direct organ-specific gene expression, we used microprojectile bombardment to introduce chimeric S-allele/&glucuronidase genes into different tissues of Petunia hybrida for transient expression. Histochemical staining showed that S-locus/B-glucuronidase fusions were expressed in pistil, ovary, and petal tissue. No expression of ...

Optimization of Transient Expression of uidA Gene in Androgenic Embryos of Wheat (Triticum aestivum L. cv. Falat) via Particle Pombardment

Haploid microspore-derived embryos (MDEs) of wheat were obtained by in vitro androgenesis. These  embryos were employed to evaluate the transient expression of GUS gene (uidA) following particle bombardment. Using the Bio-Rad PDS-1000/He system, the physical parameters including rupture disk pressure (900, 1100 and 1350 psi); microprojectile travel distance (6 and 9 cm); gold particles size (0....

Scaffold attachment regions increase reporter gene expression in stably transformed plant cells.

The yeast ARS-1 element contains a scaffold attachment region (SAR) that we have previously shown can bind to plant nuclear scaffolds in vitro. To test effects on expression, constructs in which a chimeric beta-glucuronidase (GUS) gene was flanked by this element were delivered into tobacco suspension cells by microprojectile bombardment. In stably transformed cell lines, GUS activity averaged ...

Promoter analysis in transient assays using a GUS reporter gene construct in creeping bentgrass (Agrostis palustris).

Transient expression profiles for several chimeric beta-glucuronidase (GUS) gene constructs were determined in tissues (young leaves, mature leaves and roots) of creeping bentgrass (Agrostis palustris, cv. Penn A4) following microprojectile bombardment. The constructs analyzed consisted of the uidA (GUS) reporter gene driven by four different promoters (ubiquitin 3-potato, ubiquitin corn, ubiqu...

Use of microprojectile bombardment in transient expression assays to analyze protochlorophyllide reductase gene expression in greening maize seedling leaf cells